[ Links ], 4. [ Links ], 10. Teste de biocompatibilidade dos materiais odontológicos. Sodium hypochlorite (NaClO) Sodium hypochlorite (NaOCl) CCRIS 708. Estrela C, Sydney GB, Bammann LL, Felippe Jr O. São Paulo: Artes Médicas; 1999. p 572-653. I was cleaning my toilet and mixed HCl and NaOCl and after a minute or so, I started to smell/taste chlorine gas that I'm so familiar with from using concentrated HCl in the lab. [ Links ], 13. The effectiveness of sodium … Chlorine (strong oxidant) presents antimicrobial action inhibiting bacterial enzymes leading to an irreversible oxidation of SH groups (sulphydryl group) of essential bacterial enzymes. However, the use of intracanal dressings is essential to eliminate surviving microorganisms (3,5). The saponification, amino acid neutralization and chloramination reactions that occur in the presence of microorganisms and organic tissue lead to the antimicrobial and tissue dissolution process. Dissolution of organic tissue can be verified in the saponification reaction when sodium hypochlorite degrades fatty acids and lipids resulting in soap and glycerol. 1997 Oct;10(4):597-610. doi: 10.1128/CMR.10.4.597-610.1997. Internet site: http://www.forp.usp.br/restauradora/Teses/Barbin/mestrado_barbin.html         [ Links ], 11. In: Metodologia Científica: ensino e pesquisa em odontologia. Control of microorganisms by calcium hydroxide pastes. The aim of this work is to discuss the mechanism of action of sodium hypochlorite based on its antimicrobial and physico-chemical properties. Estrela C, Bammann LL, Pimenta FC, Pecora JD. Braz Dent J 2001;12:154-157. Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Estrela C, Figueiredo JAP. Please enable it to take advantage of the complete set of features! The antimicrobial activity is related to bacterial essential enzymatic sites promoting irreversible inactivation by hydroxyl ions and the chloramination reaction. Byströn A, Sundqvist G. Bacteriologic evaluation of the effects of 0.5% sodium hypochlorite in endodontic therapy. Estrela C, Sydney gb, Bammann ll, Fellipe jr O. Estudo do efeito biológico do pH na atividade enzimática de bactérias anaeróbias. Internet site: http://estrela.neomundi.com.br/         [ Links ], 17. Periodontol 2000;1997:13:121-48. Our channel. The minimum concentration of 2% sodium hypochlorite required for inhibiting S. aureus, E. faecalis, P. aeruginosa and C. albicans was 0.2%, and 2% for B. subtilis and for the mixture. NaClO + 2HCl → Cl 2 + NaCl + H 2 O [ Check the balance ] Sodium hypochlorite react with hydrogen chloride to produce chlorine, sodium chloride and water. sodium hypochloride. Internet site: http://www.forp.usp.br/restauradora/Teses/Tanit/tanit_mestrado.html         [ Links ], 12. [ Links ], 15. Solutions without surfactant presented a decrease in surface tension and those with surfactant an increase (10); 3) with the elevation of temperature of the sodium hypochlorite solutions, dissolution of the bovine pulp tissue was more rapid (11); 4) the percent variation of the sodium hypochlorite solutions, after dissolution, was inversely proportional to the initial concentration of the solution, or in other words, the greater the initial concentration of the sodium hypochlorite solutions, the smaller was the reduction of its pH (12). COVID-19 is an emerging, rapidly evolving situation. Knowledge about morphology, structure and physiology of microorganisms that are responsible for pain and destruction of periapical tissues led to several therapeutic trends. This process is begun during root canal preparation with chemical agents and the use of an intracanal dressing that aids in success of this process (3,5). The organic dissolution action can be observed in the saponification reaction when sodium hypochlorite degrades lipids and fatty acids resulting in the formation soap and glycerol. [ Links ], 6. [Master's thesis]. Santos TC. Get the latest research from NIH: https://www.nih.gov/coronavirus. Surviving Reactive Chlorine Stress: Responses of Gram-Negative Bacteria to Hypochlorous Acid. Hypochlorous acid, a substance present in sodium hypochlorite solution, when in contact with organic tissue acts as a solvent, releases chlorine that, combined with the protein amino group, forms chloramines (scheme 3 - chloramination reaction). Sci Rep. 2020 Oct 12;10(1):17014. doi: 10.1038/s41598-020-73926-z. Its antimicrobial mechanism of action can be observed verifying its physico-chemical characteristics and its reaction with organic tissue. The chemical reactions verified between organic tissue (7,10-12) and sodium hypochlorite are shown in schemes 1-3. [ Links ], 9. In: Endodontia - Princípios biológicos e mecânicos. 108p. Sodium hypochlorite react with hydrogen chloride. [ Links ], 8. NaOCl = H 2 O « NaOH + HOCl « Na + ... Its antimicrobial mechanism of action can be observed verifying its physico-chemical characteristics and its reaction with organic tissue. Some physico-chemical characteristics must be mentioned. 1. Because enzymatic sites are located in the cytoplasmic membrane, which is responsible for essential functions such as metabolism, cellular division and growth, and take part in the last stages of cellular wall formation, biosynthesis of lipids, transport of electrons and oxidative phosphorylation, the authors believe that hydroxyl ions from calcium hydroxide develop their mechanism of action in the cytoplasmic membrane. Pecora JD, Costa WF, Campos GM, Roselino RB. Mechanism of action of calcium and hydroxyl ions of calcium hydroxide on tissue and bacteria. Rev Fac Odontol Bauru 1994:2:29-36. 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